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All mice were singly housed on corn cob bedding with a red dome and wood stick for enrichment, and provided with either 0g, 3g or 6g of shredded paper-strip nesting material. Twenty-four male C57BL/6J mice were used in this study. It is also of interest to know if providing 6g of nesting material, the minimum required to create a sufficient nest, affects mouse metabolism. Our study aims to determine the amount of nesting material that may be provided without interfering with data collection when using metabolic and behavior monitoring systems. However, social isolation in barren environments negatively affects animal welfare and can introduce unintended variation in metabolic phenotypes induced by factors such as stress and impaired thermoregulation. Most animal monitoring systems require mice to be singly housed, often for a period of several days, without environmental enrichment and nesting material to ensure that there is no interference with the collection of metabolic measurements. Metabolic and behavior monitoring systems are commonly used in laboratory animal facilities.

P1 Refinements for Mice Housed in Metabolic and Behavior Phenotyping SystemsĪ Healy* 1, C Yeung 2,3, M Choe 2, H Sung 2,3ġAnimal Resources and Compliance, The Center for Phenogenomics, Toronto, Canada 2Translational Medicine Program, The Hospital for Sick Children Research Institute, Toronto, Canada 3Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada

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